Mathematical monitoring tools to watch microbial growth for recombinant protein production in fermenters

Venkata Sri Krishna Kona; Azharuddin Mohammed; Deepthi Sri Kanigiri; Lekha Arrolla

doi:10.37022/jpmhs.v6i3.90

Authors

Kona Venkata Sri Krishna Jawaharlal Nehru Technological University, Hyderabad, TS, India
Mohammed Azharuddin Vignan University, Guntur, AP, India
Kanigiri Deepthi Sri K L University, Vijayawada, AP, India
Arrolla Lekha Sreenidhi Institute of Science and Technology, Hyderabad, TS, India

DOI:

https://doi.org/10.37022/jpmhs.v6i3.90

Keywords:

stoichiometry, thermodynamics of cellular growth, transport phenomena

Abstract

The aim of this paper is to present a mathematical monitoring tool to watch microbial growth for recombinant protein production in fermenters. The method is based on a combination of engineering knowledge--microbial phenomena that includes stoichiometry, thermodynamics of cellular growth, kinetics, and physical processes (transport phenomena) like mixing, energy use, mass, and heat transfer. The development of fermentation models is assisted by the insights gained from measurements taken throughout process operations. The models have been developed using the knowledge gained from a variety of experiments. The effect on the growth rate and productivity of the microorganism(s) in the culture. Factors to consider in the design and operation of a bioreactor, as it can affect the mixing and oxygen transfer in the fermenter.

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