Extraction of paraben from blue berries and separation by column chromatography

Sruthi N; Asma Parveen S; Prapurna Chandra Y

doi:10.37022/jpmhs.v8i1.120

Authors

N.Sruthi B. Pharmacy, IV Year, Ratnam Institute of Pharmacy, spsr Nellore, Andhra Pradesh.
S. Asma Parveen Associate professor, Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, spsr Nellore, Andhra Pradesh.
Y. Prapurna Chandra Principal of Ratnam Institute of Pharmacy, spsr Nellore, Andhra Pradesh.

DOI:

https://doi.org/10.37022/jpmhs.v8i1.120

Keywords:

Paraben extraction, blueberries, column chromatography, natural preservatives, solvent extraction, silica gel, thin layer chromatography, gradient elution, food safety, natural products analysis

Abstract

Parabens are naturally occurring phenolic compounds commonly found in fruits such as blueberries and are valued for their antimicrobial and preservative properties. This study aims to extract parabens from blueberries and purify them using column chromatography. The extraction was carried out using a methanol-water solvent system (80:20 v/v) to maximize the yield of parabens. The crude extract was filtered and concentrated before further purification. Solvent partitioning with ethyl acetate was performed to enhance the separation of parabens from other non-polar compounds. The concentrated extract was subjected to column chromatography using silica gel as the stationary phase and a hexane-ethyl acetate gradient system as the mobile phase. Fractions were collected systematically and analyzed for the presence of parabens using Thin- Layer Chromatography (TLC), with standard parabens as reference markers. Selected fractions containing parabens were further validated using High-Performance Liquid Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS) for confirmation and quantification. The results demonstrated successful extraction and isolation of parabens from blueberries, with efficient separation achieved using column chromatography. The findings can contribute to the development of natural preservatives for use in pharmaceutical, cosmetic, and food industries, offering an eco-friendly alternative to synthetic parabens.

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