Role of Interferon Gamma in the Pathogenesis of Sickle Cell Crisis
Ahmed Mahmoud Khalifa1, Bashair Mater Albeladi2, Hajar Abdulmalik Amin2, Nida Mahmoud Johari2, Rawan Sultan Alosaimi2, Wafa Ahmad Hamdi2, Amal Fathy Gharib2, Howaida Mahmoud Hagag2, Asmaa Hassan Farghly2, Khadiga Ahmed Ismail2,3*
1Forensic and Toxicology Department, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.
2Medical Laboratory Department, Faculty of Applied Medical Science, Taif University, Taif, Saudi Arabia.
3Parasitology Department, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.
Sickle cell disease (SCD) is considered as one of the major public health disease worldwide. The high burden of the disease in Saudi Arabia and Egypt need more effort to control. Current evidence suggests that available care is suboptimal. Different types of crisis are considered significant complication of SCD, the pathogenesis of SCD is mainly due to the production of pro-inflammatory cytokines. As a result of the association of chronic inflammation with crisis onset and the role of IFN gamma as regulatory cytokines, we tested the association of level of IFN gamma with pathogenesis of sickle cell crisis. Study subjects comprised of 70 volunteers (42 (60%) males and 28 (40%)) and among 70 subjects, 50 were sickle cell patients [34 (48.6%) with stable condition, 16 (22.9%) with hemolytic crisis] and 20 (28.6%) healthy normal persons (Control group). Serum IFN gamma concentrations were determined by ELISA. It was found from the results that IFN gamma levels were significantly increased in both stable condition and hemolytic crisis patients in comparison to healthy normal control. There is a statistically significant increase in the level of interferon gamma in SCD patients with stable condition and in crisis in comparison to healthy control. Interferon gamma has a role in pathogenesis of inflammation in sickle cell individuals in the steady state or in crisis in comparison to healthy normal control.
Key words: Sickle cell disease, Crisis, Interferon gamma.