https://uniquepubinternational.com/journals/index.php/jcpbs <section class="text-token-text-primary w-full focus:outline-none [--shadow-height:45px] has-data-writing-block:pointer-events-none has-data-writing-block:-mt-(--shadow-height) has-data-writing-block:pt-(--shadow-height) [&amp;:has([data-writing-block])&gt;*]:pointer-events-auto [content-visibility:auto] supports-[content-visibility:auto]:[contain-intrinsic-size:auto_100lvh] R6Vx5W_threadScrollVars scroll-mb-[calc(var(--scroll-root-safe-area-inset-bottom,0px)+var(--thread-response-height))] scroll-mt-[calc(var(--header-height)+min(200px,max(70px,20svh)))]" dir="auto" data-turn-id="request-WEB:d6f38468-9ba3-453a-825d-e967276f83e6-28" data-testid="conversation-turn-58" data-scroll-anchor="false" data-turn="assistant"> <div class="text-base my-auto mx-auto pb-10 [--thread-content-margin:var(--thread-content-margin-xs,calc(var(--spacing)*4))] @w-sm/main:[--thread-content-margin:var(--thread-content-margin-sm,calc(var(--spacing)*6))] @w-lg/main:[--thread-content-margin:var(--thread-content-margin-lg,calc(var(--spacing)*16))] px-(--thread-content-margin)"> <div class="[--thread-content-max-width:40rem] @w-lg/main:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn"> <div class="flex max-w-full flex-col gap-4 grow"> <div class="min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal outline-none keyboard-focused:focus-ring [.text-message+&amp;]:mt-1" dir="auto" tabindex="0" data-message-author-role="assistant" data-message-id="f75acf03-6c26-4330-93ff-f4b0975f2830" data-message-model-slug="gpt-5-3" data-turn-start-message="true"> <div class="flex w-full flex-col gap-1 empty:hidden"> <div class="markdown prose dark:prose-invert w-full wrap-break-word light markdown-new-styling"> <p data-start="0" data-end="433" data-is-last-node="" data-is-only-node=""><strong data-start="0" data-end="67" data-is-only-node="">Journal of Comprehensive Pharmaceutical and Biomedical Sciences</strong> is a peer-reviewed publication covering all branches of pharmaceutical and biomedical sciences. It publishes original research, reviews, and short communications on drug discovery, development, pharmacology, biotechnology, and clinical studies, promoting interdisciplinary approaches to advance therapeutic innovation, healthcare outcomes, and scientific knowledge.</p> </div> </div> </div> </div> </div> </div> </section> en-US Journal of Comprehensive Pharmaceutical and Biomedical Sciences https://uniquepubinternational.com/journals/index.php/jcpbs/article/view/229 <p>The present research focuses on the development and validation of a Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) method for the simultaneous estimation of Valproate and Lamotrigine in combined pharmaceutical dosage forms. The primary objective of this study was to establish a simple, accurate, precise, and cost-effective analytical method suitable for routine quality control and stability analysis. Chromatographic separation was achieved using a Discovery C18 column (250 × 4.6 mm, 5 µm) with a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60:40 v/v, pH was adjusted to 3 with 0.1% Ortho phosphoric acid, at a flow rate of 1.0 mL/min. Detection was performed at a wavelength of 230 nm, where both drugs exhibited significant absorbance. Under the optimized chromatographic conditions, sharp and symmetrical peaks were obtained with retention times of 2.365 minutes for Valproate and 3.423 minutes for Lamotrigine, showing excellent resolution and separation. The developed method was validated as per the International Council for Harmonisation (ICH Q2 R1) guidelines. The method demonstrated good linearity over the concentration range of 25–150 µg/mL for Valproate and 12.5–75 µg/mL for Lamotrigine, with correlation coefficients (R²) greater than 0.999. Accuracy studies showed mean recoveries between 98% and 102%, while precision results confirmed reproducibility with %RSD values below 2. The method also proved to be robust and specific, showing no interference from excipients or degradation products. Hence, the developed RP-HPLC method is rapid, reliable, and suitable for routine simultaneous analysis of Valproate and Lamotrigine in combined dosage forms.</p> Nagaraju Pappula Copyright (c) 2026 2026-08-15 2026-08-15 1 6