https://uniquepubinternational.com/journals/index.php/jcls <div class="col-lg-9"> <div class="full-width"> <div id="pl-559" class="panel-layout"> <div id="pg-559-0" class="panel-grid panel-no-style"> <div id="pgc-559-0-1" class="panel-grid-cell"> <div id="panel-559-0-1-0" class="so-panel widget widget_sow-editor panel-first-child panel-last-child" data-index="1"> <div class="so-widget-sow-editor so-widget-sow-editor-base"> <div class="siteorigin-widget-tinymce textwidget"> <p><strong>UPI Journal of Chemical and Life Sciences (UPI-JCLS)-ISSN: 2581-4648</strong> is a quarterly publishing, double blind peer reviewed, open access, international journal and covers all the subjects/departments of chemical and life sciences including but not limited to Organic Chemistry, Inorganic Chemistry, Biotechnology, Bio and Pharmacoinformatics, Microbiology, Biochemistry, Food Technology, Botany, Geochemistry, Zoology and related fields of chemical and life sciences. </p> </div> </div> </div> </div> </div> </div> </div> </div> <div class="col-lg-3"> <div class="side-bar"> <div id="search-3" class="course-info-side widget_search"> </div> </div> </div> Unique Pub International en-US UPI Journal of Chemical and Life Sciences 2581-4648 <p><strong>Copyright © Author(s) retain the copyright of this article.</strong></p> https://uniquepubinternational.com/journals/index.php/jcls/article/view/174 <p>Low aqueous solubility and concomitant oral bioavailability are the major glitches found in converting the active pharmaceutical ingredients to new pharmaceutical products. Many methods are in existence to improve the solubility of poorly water-soluble drugs. Co-crystallization is one of the unique technique that aggregates two or more different chemical entities in a crystalline lattice via non-covalent bonding. Co-crystals are multi-component system of an active pharmaceutical ingredient and a conformer. </p> Vinod Kumar K Vyshnavi K Reethi N Reethi N Siddardh H Vaikunteswar A Copyright (c) 2026 https://creativecommons.org/licenses/by-nc/4.0 2026-02-10 2026-02-10 1 4 10.37022/jcls.v9i1.174 https://uniquepubinternational.com/journals/index.php/jcls/article/view/218 <p>Modern pharmaceutical analysis increasingly relies on advanced multidimensional analytical techniques for comprehensive characterization of drug substances, drug products, biopharmaceuticals, and nanomedicine formulations. High-Performance Liquid Chromatography (HPLC), Ultra-Performance Liquid Chromatography (UPLC), UV-visible spectroscopy, and Size Exclusion Chromatography coupled with Multi-Angle Laser Light Scattering (SEC-MALLS) have emerged as highly powerful analytical platforms because of their sensitivity, selectivity, reproducibility, and broad pharmaceutical applicability. HPLC and UPLC are extensively utilized for assay determination, impurity profiling, dissolution testing, degradation studies, stability-indicating analysis, and pharmaceutical quality control, while UV-visible spectroscopy provides rapid and economical quantitative estimation of pharmaceutical compounds containing chromophoric functional groups. SEC-MALLS additionally enables absolute molecular weight determination, aggregation profiling, conformational analysis, and structural characterization of proteins, monoclonal antibodies, biosimilars, nanoparticles, liposomes, and advanced drug delivery systems without dependence on calibration standards. Integration of orthogonal analytical techniques significantly improves analytical reliability, multidimensional characterization capability, impurity identification, and structural elucidation in complex pharmaceutical formulations. The present review comprehensively discusses the principles, instrumentation, pharmaceutical applications, analytical advantages, method development strategies, validation requirements, regulatory significance, and future perspectives of HPLC, UPLC, UV-visible spectroscopy, and SEC-MALLS in modern pharmaceutical analysis. Particular emphasis is placed on multidetector analytical systems, integrated analytical workflows, pharmaceutical quality assessment, biosimilar evaluation, nanotechnology applications, and regulatory quality control. Emerging advancements involving AI-assisted analytics, automated multidetector platforms, high-resolution chromatography, and real-time process monitoring are also highlighted.</p> Venkatanarayana Bypaneni Jayaram Kamma Palanivelu Murugesan Copyright (c) 2026 https://creativecommons.org/licenses/by-nc/4.0 2026-05-31 2026-05-31 5 12